Component | 50 μL | Final Concentration | |
---|---|---|---|
1 | Nuclease–free water | 30.0 | |
2 | 5X PrimeSTAR GXL buffer | 10 | 1X |
3 | dNTP Mixture (2.5 mM each) | 4 | 200 μM |
4 | 10 μM sgRNA_forward | 2.5 | 0.5 μM |
5 | 10 μM sgRNA_reverse | 2.5 | 0.5 μM |
6 | PrimeSTAR GXL DNA Polymerase | 1.0 | 1.25 unit/50 μL reaction |
total | 50 |