| Components | 25 μL Reaction (μL) | Final Concentration | |
|---|---|---|---|
| 1 | 5× PrimeSTAR GXL Buffer | 5.0 | 1X |
| 2 | dNTP Mixture (2.5 mM each) | 2.0 | 200 μM each |
| 3 | Forward Primer (10 pmoles/μL) | 0.5 | 0.2 pmoles/μL |
| 4 | Reverse Primer (10 pmoles/μL) | 0.5 | 0.2 pmoles/μL |
| 5 | Template (crude lysate) | 1.0 | |
| 6 | PrimeSTAR GXL DNA Polymerase | 0.5 | 0.625 U/50 μL |
| 7 | Sterile Distilled Water | 15.5 | |
| Total | 25.0 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.