Fig. 4.
Effect of Hypoxia and TGF-β on the Expression of Endothelial and Smooth Muscle Markers in HCAECs. a Representative Western blot images comparing changes in the expression of the endothelial marker CD31 and the smooth muscle marker α-SMA and SM22-α in HCAECs after exposure to different doses of TGF-β1 and TGF-β2 under Normoxia (21 % O2) and Hypoxia (1 % O2). b Quantification of endothelial and smooth muscle marker expression by densitometry analysis of western blots comparing the effect of hypoxia to baseline and normoxia control. c Quantification of endothelial and smooth muscle marker expression by densitometry analysis of western blots comparing the effect of TGF-β1 and TGF-β2 under Normoxia (21 % O2) and Hypoxia (1 % O2). Results were normalized to α-Tubulin and Gapdh (loading control) and expressed as fold-change relative to baseline (BL) in B, and relative to control without TGF-β in C. (n = 3, *p < 0.05, **p < 0.005)