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. 2016 Apr 27;18:10. doi: 10.1186/s12575-016-0040-3

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© Pinto et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Effect of TGF-β on the Expression of Endothelial and Smooth Muscle Markers in HMPAECs. a Representative Western blot images comparing changes in the expression of the endothelial marker CD31 and the smooth muscle marker α-SMA and SM22-α in HMPAECs after exposure to different doses of TGF-β1 and TGF-β2 under Normoxia (21 % O₂) and Hypoxia (1 % O₂). b Quantification of endothelial and smooth muscle marker expression by densitometry analysis of western blots comparing the effect of hypoxia to baseline and normoxia control. c Quantification of endothelial and smooth muscle marker expression by densitometry analysis of western blots comparing the effect of TGF-β1 and TGF-β2 under Normoxia (21 % O₂) and Hypoxia (1 % O₂). Results were normalized to Gapdh (loading control) and expressed as fold-change relative to baseline (BL) in B, and relative to control without TGF-β in C. (n = 3–4, *p < 0.05)