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. 2016 Apr 4;5(4):e213. doi: 10.1038/oncsis.2016.18

Figure 7.

Figure 7

Expression of TRA2β4 in colon cancers. (a) HCT116 cells were transfected with pcDNA3.1 (mock), pCMV-TRA2β4 or pCMV-TRA2β4 mutated in the stem-loop motif (pCMV-TRA2β4mt, 485-AAGG-488) for 48 h. mRNA levels were measured by qPCR using GAPDH as an endogenous quantity control. *Significantly decreased compared with mock-treated cells (P<0.05 by Student's t-test). (b) After transfection of HCT116 cells (1 × 104 cells per 24-well dish) with pcDNA3.1 (mock), pCMV-TRA2β4 or pCMV-TRA2β4mt, the number of growing cells were measured using CellTiter96 AQueous Cell Proliferation Assay (MTS). Values expressed as means±s.d. (n=3). *Significantly increased compared with mock-transfected cells (P<0.05 by Student's t-test). (c) Using human colon cancer tissue qPCR arrays (TissueScan, HCRT103), TRA2β4 expressed in cDNAs from adenocarcinomas of the colon and surrounding normal colon tissues were measured by qPCR in 24 patients. Values were normalized to ACTB mRNA levels.