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. 2016 Apr 4;5(4):e214. doi: 10.1038/oncsis.2016.23

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Copyright © 2016 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Inhibition of EZH2 induced FOXO3 expression in basal-type cell lines. The basal type cell lines (a) HCC70 and (b) MDA-MB-468, as well as the luminal (c) MCF-7 cells were treated with 0, 1 and 5 μM of the EZH2 inhibitor GSK126 for 72 h with culture medium changed every day. Total protein was extracted from these cells and analysed by western blotting with the indicated antibodies. In parallel, total RNA was also extracted and expression of BRCA1, EZH2 and FOXO3 mRNA was analysed by qRT–PCR. The experiments were repeated three times independently and qRT–PCR results were normalized against L19 mRNA levels and the results expressed as mean±s.d. *P⩽0.05 and **P⩽0.001; NS, not significant Students' t-test.