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. 2016 Apr 4;5(4):e214. doi: 10.1038/oncsis.2016.23

Figure 5.

Figure 5

Depletion of EZH2 induced FOXO3 expression in HCC70 but not in MCF-7 cells. Western blotting and qRT–PCR analysis was performed on (a) HCC70 and (b) MCF-7 cells mock transfected or transfected with EZH2-specific siRNA pool or non-silencing control (NSC) siRNA pool for 48 h. Depletion of EZH2 by siRNA in HCC70 significantly induced FOXO3 expression but did not affect FOXO3 expression in MCF-7 cells. These experiments have been repeated three times and the representative western blottings were shown. qRT–PCR data were expressed as mean±s.d; *P<0.05, **P<0.01 and ***P<0.001; NS, not significant.