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. 2016 Apr 27;15:46. doi: 10.1186/s12938-016-0161-6

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© Shu et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Stain colour detection in toolbox. This is an illustration of the training phase of colour detection. The left image is the original image. The right image is the output image. The colour pixels in the output image are colours similar to the colours in the selected ROI in the original image. The background pixels are removed by using the scrolling bar, and are set to be 255 in the output image