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. 2016 Apr 27;9:94. doi: 10.1186/s13068-016-0508-z

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© Golberg et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — a Pulsed electric field effects on the Nicotiana Tabacum L. cv bright yellow-2 cells with cell wall stained with vital dye solution (Phenosafranine). The pulsed electric field protocol: E = 2. 5 kV/cm, n = 20, f (pulse frequency) = 2 Hz, exponential pulses with duration of 400 ms. b Pulsed electric fields effects on the extracellular matrix of potato. The pulsed electric field protocol: E = 5 kV/cm, n = 20, f (pulse frequency) = 2 Hz, exponential pulses with duration of 100 ms. Tissue staining was performed with ruthenium red 5 min after PEF treatment

Figure adapted from [32]