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. 2016 Apr 20;17(4):342. doi: 10.3390/ijms17040342

Figure 2.

Figure 2

Relative expression of EPSPS1 (a,b), M10 (c,d), and M11 (e,f) genes analyzed by real time PCR. Leaf samples were taken from plants grown at 24 °C. Relative quantification was obtained through the delta-delta-Ct algorithm (2ΔΔCt) method using actin as the reference gene. As a reference sample, the untreated (control) plants from the susceptible biotype (Cs) was chosen. Data represent the average from three biological replicates and the error bars indicate the standard deviation. The following plant–treatment combinations were studied: Cs susceptible biotype-untreated control; Cr resistant biotype-untreated control; S(1x) susceptible biotype- glyphosate sprayed, dose 1x; R(1x) resistant biotype-glyphosate sprayed, dose1x; S(8x) susceptible biotype-glyphosate sprayed, dose 8x; R(8x) resistant biotype-glyphosate sprayed, dose 8x. Relative expression ratios were estimated one and four days after glyphosate treatment (1 DAT—diagrams on the left and 4 DAT—diagrams on the right).