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. 2016 Apr 8;17(4):382. doi: 10.3390/ijms17040382

Figure 3.

Figure 3

Translocation of CXCR4 in HeLa cells. (A,B) The subcellular localization of hCXCR4 and hRTN3. HeLa cells were transfected with pcDNA3.1-Myc-hCXCR4 (A) and pCMV-Flag-hRTN3 (B); (C,D) Translocation and colocalization of CXCR4 and RTN3. HeLa cells were co-transfected with human Myc-CXCR4 and Flag-RTN3 (C) or with zebrafish Myc-CXCR4b and Flag-RTN3 (D). After 48 h, the transfected cells were fixed and subjected to immunofluorescence assay using anti-Myc mouse monoclonal antibody (mAb), anti-Flag mAb, and 4′,6-diamidino-2-phenylindole (DAPI). The results of subcellular localization were screened using confocal laser-scanning microscopy. Arrows indicate puncta in HeLa cells. Scale bar, 10 µm.