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. 2016 Mar 28;17(4):438. doi: 10.3390/ijms17040438

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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TOR pathway transduces AAs signaling that regulates vitellogenin (Vg) synthesis. (A) Knockdown efficiency of target genes in the fat body of the dsRNA of gfp, Rheb, TOR and S6K. The relative mRNA levels of these respective genes in control (gfp dsRNA) were set to 1 and the numbers above each bar indicated the percentage knockdown efficiency of corresponding gene; (B) Effect of the TOR pathway genes knockdown on the mRNA and protein levels of Vg (Student’s t-test, * denotes p < 0.05); (C) Effect of AAs on S6K phosphorylation. Western blot analysis was performed using antibody against the phospho-S6K (Thr-389); (D) RNAi-mediated knockdown of Rheb or TOR decreased S6K phosphorylation levels; (E) TOR inhibitor rapamycin effectively decreased S6K phosphorylation and Vg expression levels.