Figure 4.

TOR pathway works through juvenile hormone (JH) biosynthesis to regulate Vg synthesis. (A) Relative mRNA levels of jmtN in the CA and met in the fat body of adult females after knockdown of TOR pathway genes (TOR or Rheb) and rapamycin treatment; (B) Relative mRNA and protein levels of Vg in the fat body of the dsRNA of gfp, Rheb and TOR further treated with JH III. Newly emerged females were injected with dsRNA of gfp, TOR or Rheb and reared for two days. After that, the insects were topically applied with JH III or acetone and provided with artificial diets for another day (Student’s t-test, * denotes p < 0.05); (C) Protein levels of Vg in the fat body of rapamycin-treated adult females were further topically applied with JH III.