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. 2016 Mar 28;17(4):438. doi: 10.3390/ijms17040438

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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AAs, TOR, Rheb and Vg are required for ovary development and fecundity. (A) Newly emerged females were injected with dsRNA of TOR, Rheb, Vg or a control gfp and reared on artificial diets lacking AAs (−AAs) or normal diets (+AAs) for 6 days. Ovaries were dissected and photographed with a stereo microscopy SMZ18 (Nikon, Tokyo, Japan). Scale bar, 500 μm; (B) AAs deprivation and RNAi-mediated depletion of TOR or Rheb significantly reduced the numbers of laid eggs by females. Ten females were analyzed per group and three independent groups were evaluated. Since females lacking AAs laid no eggs, only egg numbers derived from the females feeding AAs (except for Vg dsRNA females) were used for statistical analysis. Student’s t-test was performed to determine significant differences in mean eggs numbers for knockdown females as compared with the gfp dsRNA-injected control group (* denotes p < 0.05).

AAs, TOR, Rheb and Vg are required for ovary development and fecundity. (A) Newly emerged females were injected with dsRNA of TOR, Rheb, Vg or a control gfp and reared on artificial diets lacking AAs (−AAs) or normal diets (+AAs) for 6 days. Ovaries were dissected and photographed with a stereo microscopy SMZ18 (Nikon, Tokyo, Japan). Scale bar, 500 μm; (B) AAs deprivation and RNAi-mediated depletion of TOR or Rheb significantly reduced the numbers of laid eggs by females. Ten females were analyzed per group and three independent groups were evaluated. Since females lacking AAs laid no eggs, only egg numbers derived from the females feeding AAs (except for Vg dsRNA females) were used for statistical analysis. Student’s t-test was performed to determine significant differences in mean eggs numbers for knockdown females as compared with the gfp dsRNA-injected control group (* denotes p < 0.05).