Table 1.

Forward and reverse primers used in real-time PCR reaction to assess mRNA expression for β-actin, heme oxygenase (HO)-1, HO-2, hypoxia inducible factor 1α (HIF-1α), inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and tumor necrosis factor α (TNF-α).

Gene	Forward Primer	Reverse Primer
β-actin	5′-GATCAAGATCATTGCTCCTCCTG-3′	5′-AGGGTGTAAAACGCAGCTCA-3′
HO-1	5′-GTCCCAGGATTTGTCCGAGG-3′	5′-GGAGGCCATCACCAGCTTAAA-3′
HO-2	5′-CCGGGCAGAAAATACCCAGT-3′	5′-ATCAGTGCTTCCTTCCGGTG-3′
HIF-1α	5′-ATCCATTTTCAGCTCAGGACAC-3′	5′-GGTAGGTTTCTGTAACTGGGTCTG-3′
iNOS	5′-TGGTGAGGGGACTGGACTTT-3′	5′-CTCCGTGGGGCTTGTAGTTG-3′
COX-2	5′-ATCAGAACCGCATTGCCTCT-3′	5′-GCCAGCAATCTGTCTGGTGA-3′
TNF-α	5′-TGGGCTCCCTCTCATCAGTT-3′	5′-TCCGCTTGGTGGTTTGCTAC-3′