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Example tandem mass spectra taken from Mascot output showing matches (numbers coloured red) to the same collagen peptide (2t69) but with (A) a variable hydroxylation matched on the 12th residue, compared with (B) a variable hydroxylation on the 14th residue (the peptide significance score for this (Bos) search was 40 and the highest false positive ion score as 31; the false discovery rate above identity threshold was 2.27%).
