Figure 2.

hAQP1 water permeability functional assay. Normalized light scattering intensity changes of hAQP1—E. coli lipid proteoliposomes (blue trace) compared with no-AQP liposome control (black trace) in response to an osmotic shock, and inhibition of hAQP1 proteoliposomes by mercuric chloride (red trace). The average fitted rate constants (k) (means ± S.E., n = 6) measured at 23 °C for hAQP1-proteoliposomes, proteoliposomes in presence of 300 µM HgCl₂ and no-AQP liposomes were 109.4 (±1.9), 8.56 (±0.03) and 2.27 (±0.04) s⁻¹, respectively.