Figure 3.

Fragment screening against hAQP1 protein using Surface plasmon resonance (SPR). Screening data adjusted from response unit to percentage surface occupancy, based on equilibrium binding response values (R_eq) obtained from the Biacore T-200 instrument for the 1500-fragment library. Each cycle number represents a different fragment. Each color group represents individual screening plates (total of 19). Screening proceeded for 15 days, and data obtained per screening plate was adjusted so that the baseline scattered evenly around zero percent occupancy. The relative binding occupancy of the positive control, NSC624169, injected at 200 µM every 20 cycles, is also shown (black triangles).