Table 1.
Proteomic identification of oxidatively-modified serum proteins at three months, six months, and 12 months of age from 3×Tg-AD mice compared with the aged-matched controls.
| Age of Mice | Spot Number | Protein Identified | SwissProt Accession | Mw (kDa)/pI | Protein Score | Peptides Matched a | Coverage Rate (%) b | Specific Oxidation (Fold) c |
|---|---|---|---|---|---|---|---|---|
| 3 months | 1 | Ig gamma-2B chain C region (IGH-3) | P01867 | 50/6.1 | 122 | 1 (1) | 3 | 3.42 |
| 2 | Ig lambda-2 chain C region (IGLC2) | P01844 | 11.4/5.86 | 216 | 3 (3) | 47 | 3.69 | |
| 3 | Ig kappa chain V-V region HP R16.7 | P01644 | 12/7.97 | 255 | 3 (3) | 43 | 4.98 | |
| 4 | Ig kappa chain V-V region HP R16.7 d | P01644 | 12/7.97 | 365 | 4 (4) | 43 | 2.1 | |
| 5 | Ig kappa chain C region (IGKC) | P01837 | 12/5.23 | 132 | 2 (2) | 28 | 4.22 | |
| 6 months | 1 | Ig gamma-2B chain C region (IGH-3) | P01867 | 50/6.1 | 122 | 1 (1) | 3 | 3.13 |
| 12 months | 1 | Ig gamma-2B chain C region (IGH-3) | P01867 | 50/6.1 | 122 | 1 (1) | 3 | 5.3 |
a Peptides matched by mass fingerprinting; b protein sequence coverage; c match between 3×Tg-AD mice and the control (3×Tg-AD vs. CTR), p < 0.05; d the Spots 3 and 4 were identified as the same protein.