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Kinetic parameters for the purified carboxylesterases towards the 1-naphthyl acetate.
| Enzyme | Specific Activity | Km | kcat |
|---|---|---|---|
| pET32a-001D | 2.24(0.14) | 7.61(0.63) | 2.29(0.08) |
| pE1-001D | 0.34(0.14) | 19.72(6.69) | 0.35(0.05) |
| pET30a-001D | 0.57(0.07) | 12.51(2.14) | 0.60(0.06) |
Specific activities (at 200 µM substrate) (μM·s−1·(μM−1·protein)) and estimates of Km (µM) and kcat (s−1) are shown. Estimates are based on an average of three replicates, and standard errors for these estimates are given in brackets.
