Table 1.

RcLPAT2 copy number in primary transgenic lines and hydroxy fatty acid (HFA) composition of T₁ seeds.

HFA Content		18:1OH	18:2OH	20:1OH	20:2OH	Total HFA
Wild type		1.20 ± 0.09	0.24 ± 0.06	51.74 ± 0.98	3.07 ± 0.39	56.18 ± 0.48
Transgenics
ID	Copy number
Line 1	2	3.79 ± 0.04 ***	0.53 ± 0.04 **	45.64 ± 1.32 *	3.11 ± 0.15	53.08 ± 1.46
Line 2	1	1.74 ± 0.08 *	0.30 ± 0.08	51.43 ± 0.50	3.45 ± 0.21	56.91 ± 0.33
Line 3	1	4.08 ± 0.31 ***	0.74 ± 0.12 *	48.99 ± 0.69	3.60 ± 0.11	57.81 ± 0.34
Line 4	2	3.81 ± 0.06 ***	0.60 ± 0.03 **	50.02 ± 0.48	3.38 ± 0.24	57.81 ± 0.34
Line 5	1	3.97 ± 0.13 ***	0.75 ± 0.20 *	48.35 ± 1.88	3.58 ± 0.31	56.64 ± 1.27
Line 7	1	3.29 ± 0.12 ***	0.34 ± 0.03	51.09 ± 0.22	2.84 ± 0.15	57.56 ± 0.21
Line 8	1	1.42 ± 0.08 **	0.15 ± 0.07	48.24 ± 1.41	2.75 ± 0.20	52.56 ± 1.26
Line 9	1	2.87 ± 0.21 ***	0.53 ± 0.07 *	53.51 ± 0.33	3.53 ± 0.31	60.44 ± 0.36 **
Line 10	1	2.66 ± 0.12	0.43 ± 0.06 *	48.98 ± 1.11	3.09 ± 0.06	55.17 ± 1.00
Line 11	1	1.42 ± 0.08	0.23 ± 0.04	49.57 ± 0.46	3.28 ± 0.09	54.50 ± 0.31 *
Line 12	1	1.35 ± 0.05	0.15 ± 0.03	51.40 ± 1.75	3.26 ± 0.26	56.15 ± 1.58
Line 13	1	2.08 ± 0.11 **	0.29 ± 0.08	47.43 ± 2.07	3.20 ± 0.49	53.00 ± 2.07
Line 14	1	1.97 ± 0.07 **	0.14 ± 0.07	53.09 ± 0.82	2.00 ± 0.13	57.19 ± 0.75
Line 15	1	1.99 ± 0.10 **	0.24 ± 0.13	52.62 ± 0.70	2.93 ± 0.26	57.77 ± 0.47
Line 16	2	1.48 ± 0.04	0.28 ± 0.03	48.96 ± 1.07	3.94 ± 0.10	54.66 ± 1.12
Line 17	2	1.42 ± 0.04	0.23 ± 0.04	51.18 ± 0.59	3.61 ± 0.26	56.44 ± 0.41
Average among lines		2.46 ± 0.26	0.37 ± 0.05	50.03 ± 0.54	3.22 ± 0.11	56.08 ± 0.54

Each HFA composition was measured as percentage of total fatty acids. Triplicates of 10-seed samples were measured for wild type and each independent transgenic line. All data are averages of three measurements ± SE. Fatty acid legend: 18:1OH is ricinoleic; 18:2OH is densipolic; 20:1OH is lesquerolic; and 20:2OH is auricolic acid. Two-tailed student’s t-test. * p < 0.05; ** p < 0.01; *** p < 0.001.