Table 1.
Optimized multiple-reaction monitoring (MRM) conditions for eicosanoid standards.
| Compounds | Abbreviation | Ion Mode | MRM Transitions | DP | CE | |
|---|---|---|---|---|---|---|
| Q1 | Q3 | |||||
| Prostaglandin E2-d4 | PGE2-d4 | Negative | 355 | 275 | −50 | −25 |
| Prostaglandin D2-d4 | PGD2-d4 | Negative | 355 | 275 | −50 | −25 |
| Leukotriene B4-d4 | LTB4-d4 | Negative | 339 | 197 | −70 | −22 |
| 14,15 Epoxy-eicosatrienoic acid-d11 | 14,15 EET-d11 | Negative | 330 | 202 | −50 | −15 |
| 5-Hydroxy-eicosatetraenoic acid-d8 | 5(S)-HETE-d8 | Negative | 327 | 116 | −50 | −20 |
| Arachidonic acid-d8 | AA-d8 | Negative | 311 | 267 | −80 | −20 |
Q1, precursor m/z; Q3, product m/z; DP, Declustering potential; CE, Collision energy.