Table 3.
Eicosanoids identified in RAW264.7 cells.
| No. | Compound Name | Abbreviation | MRM Transitions | DP | CE | RT (min) | IS | Alteration by LPS Treatment | |
|---|---|---|---|---|---|---|---|---|---|
| Q1 | Q3 | ||||||||
| 1 | 12S-hydroxy-heptadecatrienoic acid | 12-HHT | 279 | 163 | −30 | −30 | 14.3 | 5(S)HETE-d8 | Up |
| 2 | 13-hydroxy-g-octadecatrienoic acid | 13-HOTre-g | 293 | 193 | −70 | −20 | 15.5 | 5(S)HETE-d8 | Up |
| 3 | 9-hydroxy-octadecadienoic acid | 9-HODE | 295 | 171 | −60 | −25 | 16.6 | 5(S)HETE-d8 | Up |
| 4 | 13-hydroxy-octadecadienoic acid | 13-HODE | 295 | 195 | −60 | −25 | 16.3 | 5(S)HETE-d8 | – |
| 5 | 9,10-hydroxy-octadecadienoic acid | 9,10-diHOME | 313 | 201 | −60 | −30 | 14.2 | 5(S)HETE-d8 | – |
| 6 | 12,13-hydroxy-octadecadienoic acid | 12,13-diHOME | 313 | 183 | −60 | −30 | 13.5 | 5(S)HETE-d8 | – |
| 7 | 9-hydroxy-eicosapentaenoic acid | 9-HEPE | 317 | 149 | −75 | −20 | 15.7 | 5(S)HETE-d8 | Up |
| 8 | 5-hydroxy-eicosapentaenoic acid | 5-HEPE | 317 | 115 | −40 | −17 | 16.6 | 5(S)HETE-d8 | Down |
| 9 | 15-hydroxy-eicosapentaenoic acid | 15-HEPE | 317 | 219 | −60 | −20 | 15.5 | 5(S)HETE-d8 | Up |
| 10 | 8-hydroxy-eicosapentaenoic acid | 8-HEPE | 317 | 127 | −70 | −25 | 15.5 | 5(S)HETE-d8 | – |
| 11 | 11-hydroxy-eicosapentaenoic acid | 11-HEPE | 317 | 121 | −70 | −24 | 15.7 | 5(S)HETE-d8 | Up |
| 12 | 12-hydroxy-eicosapentaenoic acid | 12-HEPE | 317 | 179 | −70 | −20 | 15.9 | 5(S)HETE-d8 | – |
| 13 | 18-hydroxy-eicosapentaenoic acid | 18-HEPE | 317 | 215 | −60 | −20 | 15.1 | 5(S)HETE-d8 | – |
| 14 | 11-hydroxy-eicosatetraenoic acid | 11-HETE | 319 | 167 | −60 | −20 | 17.1 | 5(S)HETE-d8 | Up |
| 15 | 9-hydroxy-eicosatetraenoic acid | 9-HETE | 319 | 151 | −60 | −20 | 17.0 | 5(S)HETE-d8 | Up |
| 16 | 5-hydroxy-eicosatetraenoic acid | 5-HETE | 319 | 115 | −60 | −20 | 17.9 | 5(S)HETE-d8 | Down |
| 17 | 8-hydroxy-eicosatetraenoic acid | 8-HETE | 319 | 155 | −60 | −20 | 17.3 | 5(S)HETE-d8 | – |
| 18 | 15-hydroxy-eicosatetraenoic acid | 15-HETE | 319 | 219 | −50 | −15 | 16.7 | 5(S)HETE-d8 | Up |
| 19 | 12-hydroxy-eicosatetraenoic acid | 12-HETE | 319 | 179 | −60 | −20 | 17.3 | 5(S)HETE-d8 | Up |
| 20 | 18-hydroxy-eicosatetraenoic acid | 18-HETE | 319 | 261 | −80 | −25 | 15.9 | 5(S)HETE-d8 | – |
| 21 | 17-hydroxy-eicosatetraenoic acid | 17-HETE | 319 | 247 | −80 | −25 | 15.9 | 5(S)HETE-d8 | – |
| 22 | 16-hydroxy-eicosatetraenoic acid | 16-HETE | 319 | 189 | −80 | −25 | 16.0 | 5(S)HETE-d8 | – |
| 23 | 5-hydroxy-eicosatrienoic acid | 5-HETrE | 321 | 115 | −70 | −19 | 19.1 | 5(S)HETE-d8 | Down |
| 24 | 15-hydroxy-eicosatrienoic acid | 15-HETrE | 321 | 221 | −70 | −21 | 17.4 | 5(S)HETE-d8 | Up |
| 25 | 5,6-dihydroxy-eicosatrienoic acid | 5,6-DHET | 337 | 145 | −75 | −25 | 16.5 | 5(S)HETE-d8 | Down |
| 26 | 8,9-dihydroxy-eicosatrienoic acid | 8,9-DHET | 337 | 127 | −60 | −30 | 15.5 | 5(S)HETE-d8 | – |
| 27 | 11,12-dihydroxy-eicosatrienoic acid | 11,12-DHET | 337 | 167 | −60 | −25 | 15.8 | 5(S)HETE-d8 | Up |
| 28 | 8-hydroxy-docosahexaenoic acid | 8-HDoHE | 343 | 109 | −70 | −20 | 17.5 | 5(S)HETE-d8 | – |
| 29 | 7-hydroxy-docosahexaenoic acid | 7-HDoHE | 343 | 141 | −60 | −18 | 17.3 | 5(S)HETE-d8 | – |
| 30 | 4-hydroxy-docosahexaenoic acid | 4-HDoHE | 343 | 101 | −70 | −17 | 18.2 | 5(S)HETE-d8 | – |
| 31 | 10-hydroxy-docosahexaenoic acid | 10-HDoHE | 343 | 181 | −60 | −17 | 16.9 | 5(S)HETE-d8 | – |
| 32 | 11-hydroxy-docosahexaenoic acid | 11-HDoHE | 343 | 149 | −60 | −19 | 17.0 | 5(S)HETE-d8 | Up |
| 33 | 13-hydroxy-docosahexaenoic acid | 13-HDoHE | 343 | 221 | −60 | −17 | 16.7 | 5(S)HETE-d8 | Up |
| 34 | 16-hydroxy-docosahexaenoic acid | 16-HDoHE | 343 | 233 | −75 | −19 | 16.5 | 5(S)HETE-d8 | Up |
| 35 | 20-hydroxy-docosahexaenoic acid | 20-HDoHE | 343 | 241 | −60 | −20 | 16.3 | 5(S)HETE-d8 | – |
| 36 | 17-hydroxy-docosahexaenoic acid | 17-HDoHE | 343 | 245 | −60 | −20 | 16.5 | 5(S)HETE-d8 | Up |
| 37 | 14-hydroxy-docosahexaenoic acid | 14-HDoHE | 343 | 205 | −60 | −18 | 16.7 | 5(S)HETE-d8 | – |
| 38 | Arachidonic acid | AA | 303 | 259 | −80 | −20 | 21.6 | AA-d8 | Down |
| 39 | Eicosapentaenoic acid | EPA | 301 | 257 | −65 | −15 | 20.4 | AA-d8 | – |
| 40 | Adrenic acid | ADA | 331 | 287 | −80 | −20 | 22.3 | AA-d8 | – |
| 41 | Dohexacosaenoic acid | DHA | 327 | 283 | −95 | −20 | 21.3 | AA-d8 | – |
| 42 | 13-oxo-octadecadienoic acid | 13-oxoODE | 293 | 113 | −70 | −30 | 16.6 | 14,15 EET-d11 | – |
| 43 | 9,10-epoxy-octadecenoic acid | 9,10-EpOME | 295 | 171 | −60 | −25 | 18.4 | 14,15 EET-d11 | – |
| 44 | 12,13-epoxy-octadecenoic acid | 12,13-EpOME | 295 | 195 | −60 | −25 | 18.1 | 14,15 EET-d11 | – |
| 45 | 5-5-oxo-eicosatetraenoic acid | 5-oxoETE | 317 | 203 | −40 | −25 | 18.3 | 14,15 EET-d11 | – |
| 46 | 15-5-oxo-eicosatetraenoic acid | 15-oxoETE | 317 | 113 | −40 | −25 | 16.7 | 14,15 EET-d11 | – |
| 47 | 11,12-epoxy-eicosatrienoic acid | 11,12-EET | 319 | 167 | −60 | −20 | 18.6 | 14,15 EET-d11 | Down |
| 48 | 14,15-epoxy-eicosatrienoic acid | 14,15-EET | 319 | 219 | −50 | −15 | 18.1 | 14,15 EET-d11 | – |
| 49 | 5,6-epoxy-eicosatrienoic acid | 5,6-EET | 319 | 191 | −30 | −20 | 18.8 | 14,15 EET-d11 | Down |
| 50 | 15-oxo-eicosadienoic acid | 15-oxoEDE | 321 | 113 | −100 | −32 | 18.1 | 14,15 EET-d11 | Up |
| 51 | Hepoxilin B3 | HXB3 | 335 | 183 | −40 | −20 | 15.5 | 14,15 EET-d11 | – |
| 52 | 19,20-epoxy Docosapentaenoic acid | 19,20-EpDPE | 343 | 241 | −60 | −20 | 17.6 | 14,15 EET-d11 | – |
| 53 | Lipoxin B4 | LXB4 | 351 | 221 | −80 | −25 | 8.4 | LTB4-d4 | Up |
| 54 | 20-carboxy-Leukotriene B4 | 20cooh LTB4 | 365 | 195 | −60 | −25 | 6.4 | LTB4-d4 | – |
| 55 | 15-deoxy-Prostaglandin A2 or 15-deoxy-Δ12,14-PGJ2 | 15d-PGA2 or 15d-PGJ2 | 315 | 271 | −50 | −15 | 15.2 | PGE2-d4 | Up |
| 56 | Tetranor-Prostanglin F Metabolite | tetranor-PGFM | 329 | 293 | −40 | −25 | 3.1 | PGE2-d4 | – |
| 57 | Prostaglandin A2 or Prostaglandin B2 or Prostaglandin J2 | PGA2 or PGB2 or PGJ2 | 333 | 271 | −30 | −20 | 10.5 | PGE2-d4 | Up |
| 58 | 15-deoxy-Δ12,14-PGD2 | 15d-PGD2 | 333 | 271 | −30 | −20 | 12.8 | PGE2-d4 | Up |
| 59 | Prostaglandin D3 | PGD3 | 349 | 269 | −55 | −25 | 7.4 | PGE2-d4 | Up |
| 60 | Prostaglandin E3 | PGE3 | 349 | 269 | −55 | −25 | 7.1 | PGE2-d4 | Up |
| 61 | 15-keto-Prostaglandin E2 | 15k PGE2 | 349 | 113 | −35 | −30 | 8.3 | PGE2-d4 | – |
| 62 | Prostaglandin K2 | PGK2 | 349 | 205 | −50 | −30 | 8.3 | PGE2-d4 | Up |
| 63 | 15-keto-Prostaglandin F2 | 15k PGF2 | 351 | 113 | −40 | −35 | 8.6 | PGE2-d4 | Up |
| 64 | Prostaglandin E2 | PGE2 | 351 | 271 | −50 | -25 | 8.1 | PGE2-d4 | Up |
| 65 | Prostaglandin D2 | PGD2 | 351 | 271 | −50 | −25 | 8.4 | PGE2-d4 | Up |
| 66 | 13,14-dihydro-15-keto Prostaglandin E2 | dhk PGE2 | 351 | 207 | −40 | −25 | 8.4 | PGE2-d4 | Up |
| 67 | 13,14-dihydro-15-keto Prostaglandin D2 | dhk PGD2 | 351 | 207 | −40 | −25 | 9.3 | PGE2-d4 | Up |
| 68 | Prostaglandin F2α | PGF2α | 353 | 193 | −50 | −30 | 8.5 | PGE2-d4 | Up |
| 69 | 15-keto-Prostaglandin F1α | 15k PGF1α | 353 | 113 | −50 | −35 | 3.1 | PGE2-d4 | – |
| 70 | 11β-13,14-dihydro-15-keto-Prostaglandin F2α | 11β dhk PGF2α | 353 | 113 | −50 | −35 | 9.3 | PGE2-d4 | Up |
| 71 | Prostaglandin E1 | PGE1 | 353 | 273 | −55 | −25 | 8.1 | PGE2-d4 | Up |
| 72 | Prostaglandin D1 | PGD1 | 353 | 273 | −55 | −25 | 8.5 | PGE2-d4 | Up |
| 73 | Prostaglandin F1α | PGF1α | 355 | 293 | −75 | −30 | 8.2 | PGE2-d4 | Up |
| 74 | 13,14-dihydro-Prostaglandin F2α | dh PGF2α | 355 | 275 | −40 | −25 | 8.9 | PGE2-d4 | – |
| 75 | Dihomo-Prostaglandin J2 | Dihomo-PGJ2 | 361 | 299 | −55 | −25 | 13.0 | PGE2-d4 | Up |
| 76 | Dihomo-15-deoxy-Prostaglandin J2 | Dihomo-15d PGJ2 | 361 | 299 | −55 | −25 | 14.4 | PGE2-d4 | Up |
| 77 | Thromboxane B3 | TXB3 | 367 | 169 | −50 | −25 | 6.4 | PGE2-d4 | – |
| 78 | Dihomo-Prostaglandin F2α | Dihomo-PGF2α | 381 | 221 | −75 | −35 | 10.0 | PGE2-d4 | Up |
| 79 | Dihomo-Prostaglandin D2 | Dihomo-PGD2 | 379 | 299 | −65 | −30 | 10.4 | PGE2-d4 | Up |