Figure 5.

IL-17A induces adipokine expression in adipocytes. (A,B) Mouse 3T3-L1 pre-adipocytes were differentiated into mature adipocytes by 10-day differentiation culture; pictures are representatives of Oil Red O staining; original magnification, 100×, with the highlighted regions (small blue boxes) taken at 200× magnification (big blue boxes); (C–E) the pre-adipocytes and adipocytes were treated without (−) or with (+) 20 ng/mL IL-17A for 2 h; mRNA expression of each gene was analyzed with qRT-PCR; the pre-adipocytes without IL-17A treatment served as the basal level for comparison; data represent mean ± SEM (error bars) of six independent experiments (n = 6 per group), * p < 0.05 between the indicated groups.