Table 3.
PCR primer sequences.
| Gene | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|
| Tnf-a | CTACTCCCAGGTTCTCTTCAA | GCAGAGAGGAGGTTGACTTTC |
| Il-6 | CCACTTCACAAGTCGGAGGCTTA | GCAAGTGCATCATCGTTGTTCATAC |
| Il-1β | TCTTCTTTGGGTATTGCTTGG | TGTAATGAAGACGGCACACC |
| Cox-2 | TGGAAAAGGTTCTTCTACGGAG | TGAACCCAGGTCCTCGCT |
| Il-17ra | CGGAGAATTAGTCCCTGTGTTG | GAACAGTCACTTCATACTCCTGG |
| Il-17rc | TTCTGCGGTATTTGACTGTTTCG | GTCCCGGACTTCAAGACCC |
| Cxcl1 | CACCCAAACCGAAGTCATAG | AAGCCAGCGTTCACCAGA |
| Ccl2 | GCCTGCTGTTCACAGTTGC | TGTATGTCTGGACCCATTCCT |
| Ccl20 | GTCCAATTCCATCCCAAAAA | AACTGGGTGAAAAGGGCTGT |
| Acsl1 | GATCTGGTGGAACGAGGCAA | CTTCGGGTTCTGGAGGCTTG |
| Atgl | CAGCACATTTATCCCGGTGTAC | AAATGCCGCCATCCACATAG |
| Dio2 | GTCCGCAAATGACCCCTTT | CCCACCCACTCTCTGACTTTC |
| Glut1 | TCAACACGGCCTTCACTG | CACGATGCTCAGATAGGACATC |
| Glut4 | TTGGCTCCCTTCAGTTTGG | CTACCCAGCCACGTTGCAT |
| Nnmt | AGGAACCAGGAGCCTTTGACT | CCTGAGGGCAGTGCGATAGG |
| Ucp1 | GGCTGGTGGTGGTCGGAGAT | CCGAAGGCAGAAGTGAAGTG |
| Pgc1a | AGACAAATGTGCTTCGAAAAAGAA | GAAGAGATAAAGTTGTTGGTTTGGC |
| Acox1 | GCCCAACTGTGACTTCCATTAA | GTAGCACTCCCCTCGAGTGAT |
| Leptin | CTGCCCCCCAGTTTGATG | GCCAGGCTGCCAGAATTG |
| Adiponectin | AACTTGTGCAGGTTGGATGG | GCCCTTCAGCTCCTGTCATT |
| Ap2 | ACACCGAGATTTCCTTCA AACTG | CCATCTAGGGTTATG TGCTCTTCA |
| Gapdh | TGCACCACCAACTGCTTAG | GGATGCAGGGATGATGTTC |