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. 2016 Apr 8;17(4):535. doi: 10.3390/ijms17040535

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons by Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Translational inhibition of C/EBPβ by miR-155. (a) Schematic drawing of the C/EBPβ transcript. Sequence alignment and evolutionary conservation between miR-155 and its theoretical binding sites in the 3′UTR of C/EBPβ mRNA from several indicated species, with sequences recognized by miR-155 seed sequence shown in bold. The sequence of C/EBPβ 3′UTR mutant used for reporter assay is also shown (asterisk); (b) luciferase reporter constructs containing 3′UTR (psiCHECK2-C/EBPβ 3′UTR) or mutant 3′UTR (psiCHECK2-C/EBPβ 3′UTR-mut) of C/EBPβ gene was cotransfected with miR-155 or miR-155 NC in HEK 293T cell and the luciferase activities (Renilla/firefly) were assayed; (c) Western blot analysis of C/EBPβ in RAW 264.7 cells; (d) integrated band densities were obtained by scanning using a densitometer. Data are representative of three independent experiments. Results shown are the mean ± SEM. * p < 0.05.