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. 2016 Apr 8;17(4):535. doi: 10.3390/ijms17040535

Figure 5.

Figure 5

miR-155 promote M.m survival in RAW 264.7 cells by inhibition of C/EBPβ and NO. (a) Western blot confirmation of shRNA-mediated knockdown of C/EBPβ in RAW 264.7 cell; (b) the effect of C/EBPβ on NOS2 expression in RAW 264.7 cells. Cells were infected with either LV-copGFP-scramble or LV-copGFP-shRNA-C/EBPβ. After 48 h, cells were infected with IFN-γ/M.m for 24 h and cell lysates were analyzed via immunoblot; (c) RAW 264.7 cells infected with either LV-copGFP-scramble or LV-copGFP-shRNA-C/EBPβ were treated with IFN-γ and infected with M.m for the indicated times, and intracellular M.m survival was quantified by CFU test at the indicated times; (d,e) RAW 264.7 cells infected with either LV-copGFP-scramble or LV-copGFP-shRNA-C/EBPβ were respectively transfected with miR-155 NC, miR-155 (d), anti-miR-155 NC or anti-miR-155 (e), followed by infection with IFN-γ/M.m for 96 h, and intracellular M.m survival was determined by CFU assay. All data are shown as the means ± SEM of three independent experiments, * p < 0.05, ** p < 0.01.