Table 1.
Use | Sequences (5′–3′) |
---|---|
C/EBPβ 3′UTR | Forward: CCGCTCGAGGCCCTGAGTAATCACTTAAAGATGTTC |
Revese: ATAAGAATGCGGCCGCAATGTCTTCACTTTAATGCTCGAAA | |
C/EBPβ 3′UTR-mut Mutant | Forward: AAGTGAAGACATTGCGGCCGCTGG |
Reverse: AATTCGTCGAAACGGAAAAGGTTCTCAAAATATACA | |
C/EBPβ shRNA | Forward: gatccCACCCTGCGGAACTTGTTCAATTCAAGAGATTGAACAAGTTCCGCAGGGTGtttttg |
Reverse: aattcaaaaaCACCCTGCGGAACTTGTTCAATCTCTTGAATTGAACAAGTTCCGCAGGGTGg | |
Scramble shRNA | Forward: GATCCTTCTCCGAACGTGTCACGTTTCAAGAGAACGTGACACGTTCGGAGAATTTTTG |
Reverse: aattcaaaaaTTCTCCGAACGTGTCACGTTCTCTTGAAACGTGACACGTTCGGAGAAg | |
mmu-miR-155 | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCCT |
Forward: CGCCTGTTAATGCTAATTGTGA | |
Revese: AGTGCAGGGTCCGAGGTAT | |
hsa-miR-155 | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACACCCCT |
Forward: CGGTTAATGCTAATCGTGATAGG | |
Reverse: CAGTGCAGGGTCCGAGGTAT | |
mmu-miR-146a | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACCCA |
Forward: GCCTGAGAACTGAATTCCATG | |
Revese: AGTGCAGGGTCCGAGGTAT | |
mmu-miR-125b | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACTCACAA |
Forward: TCCCTGAGACCCTAACTTGTGAG | |
Revese: CAGTGCAGGGTCCGAGGTAT | |
mmu-miR-129 | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCAAGC |
Forward: CTTTTTGCGGTCTGGGCTT | |
Revese: CAGTGCAGGGTCCGAGGTAT | |
Let-7 | RT: GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAACTAT |
Forward: TGAGGTAGTAGGTTGTATAGTTGTCGTAT | |
Revese: CAGTGCAGGGTCCGAGGTAT | |
RNU6-1 | RT: AACGCTTCACGAATTTGCGT |
Forward: CTCGCTTCGGCAGCACA | |
Revese: AACGCTTCACGAATTTGCGT |
RT: reverse transcription primer of first-strand cDNA synthesis; lowercase letters is restriction enzyme cutting site.