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. 2016 Apr 22;17(4):600. doi: 10.3390/ijms17040600

Figure 4.

Figure 4

Reverse Transcription-Polymerase Chain Reaction (RT-PCR) amplicons produced on the basis of cDNA of the variety “Sebastian” (A); and the homozygous mutant brd1-d (B); RT-PCR reaction on cDNA of the heterozygote WT(Sebastian)/brd1-d producing double bands (C); the heterozygote shows normal phenotype. RT-PCR reaction without reverse transcriptase was used as a negative control (D). RT-PCR amplification of the barley actin mRNA (NCBI GenBank: AY145451) was performed as a positive control. SM—size marker, GeneRuler DNA Ladder Fermentas (100 bp), 1.5% agarose gel.