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. 2016 Apr 22;17(4):611. doi: 10.3390/ijms17040611

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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Yeast one-hybrid assays of AaDREB1. All yeast transformants carrying dual reporter genes were examined for growth in the presence of 3-AT, and β-galactosidase activity. The pAD-AaDREB1 was transformed into DRE yeast (D1, D2), with the DRE yeast containing the pAD-AtCBF3 as the positive control (D+) and the mDRE yeast containing the pAD-AtCBF3 as the negative control (D-). Transformants were streaked on YPAD medium or SD/-Try with 30 mM 3-AT and grown at 30 °C for 2 days to observe growth and β-galactosidase activities. Left panel, YPAD medium; middle panel, SD/-Try medium containing 30 mM 3-AT for growth analysis; right panel, β-galactosidase activity analysis results.