Figure 4.

Heparin has no effect on recombinant RANKL-induced osteoclast formation. (A) RAW264.7 cells were monocultured for six days, with or without 50 ng/mL recombinant RANKL and 10 IU/mL heparin. The osteoclasts were stained for TRAP (80×); (B–D) RAW 264.7 cells were monocultured under stimulation of 50 ng/mL recombinant RANKL, with addition of different level of heparin. Osteoclast number, pit area, and giant osteoclast number were quantified in each group. No significantly different among each group (n = 5). MNC: multinuclear cell; TRAP: tartrate-resistant acid phosphatase; RANKL: receptor activator of NFκB ligand; Each of the above values is expressed as the mean ± S.D.