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. 2016 Apr 22;17(4):613. doi: 10.3390/ijms17040613

Figure 5.

Figure 5

Characteristics of heparin’s effect on osteoclast formation. (A) Osteoclast numbers in a co-culture of RAW264.7 and MLO-Y4, treated with higher level of heparin. When heparin concentration is over 250 IU/mL, no significance among each group (n = 3, p = 0.993); (B) RAW264.7 were monocultured and induced with 50 ng/mL RANKL, with or without replenishment of 10 ng/mL exogenous OPG. The effects of heparin treatment on osteoclast formation were quantified (n = 3); (C) The number of osteoclasts in co-cultured system supplemented with various concentration of OPG. MNC: multinuclear cell; TRAP: tartrate-resistant acid phosphatase; RANKL: receptor activator of NFκB ligand; Each of the above values is expressed as the mean ± S.D. * p < 0.05 represent significant differences from the relevant group 0.