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. 2016 Apr 23;17(4):616. doi: 10.3390/ijms17040616

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© 2016 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).

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ROS are involved in the piperlongumine-mediated caspase-dependent apoptosis in human OSCC cells. The cell viability of (A) OC2 and (B) OCSL cells treated with piperlongumine in the presence or absence of N-acetyl-L-cysteine (NAC, inhibitor of ROS) was measured by CCK-8 analysis after 48 h post-treatment; (C) OC2 and (D) OCSL cells were treated with DMSO, piperlongumine in the presence or absence of NAC for 24 and 48 h, and the apoptotic cells were determined with flow cytometry after PI-annexin-V double staining; (E) The activation of caspase-3 and PARP-1 was detected under PL in the presence or absence of NAC treatment for 24 and 48 h by Western blotting. Data are presented as the mean ± SD. ** p < 0.01; *** p <0.001.

ROS are involved in the piperlongumine-mediated caspase-dependent apoptosis in human OSCC cells. The cell viability of (A) OC2 and (B) OCSL cells treated with piperlongumine in the presence or absence of N-acetyl-L-cysteine (NAC, inhibitor of ROS) was measured by CCK-8 analysis after 48 h post-treatment; (C) OC2 and (D) OCSL cells were treated with DMSO, piperlongumine in the presence or absence of NAC for 24 and 48 h, and the apoptotic cells were determined with flow cytometry after PI-annexin-V double staining; (E) The activation of caspase-3 and PARP-1 was detected under PL in the presence or absence of NAC treatment for 24 and 48 h by Western blotting. Data are presented as the mean ± SD. ** p < 0.01; *** p <0.001.