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. 2016 Feb 4;7(2):e2086. doi: 10.1038/cddis.2016.10

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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MiR-222 overexpression and Rbm24 silencing inhibit myoblast fusion. (a) MSC myoblasts transfected in GM with siGFP, miR-222 or siRbm24 duplex RNAs were maintained in GM for 1 day (GM) or shifted to DM for 1 day (1d) or 2 days (2d). Cells were subjected to immunofluorescence with antibodies specific for skeletal Myosin Heavy Chain (MHC) and nuclei were counterstained with Hoechst dye. Scale bar: 100 μm. (b) Quantitation of fusion and MHC-positive (MHC⁺) cells in DM at 1d and 2d in at least three independent experiments (averages±S.E.) is shown in the table. The percentage of fusion was calculated as the ratio of nuclei in MHC⁺ myotubes containing three or more nuclei over total nuclei given as 100% at least 500 nuclei were counted for each plate