Figure 1.

Synergistic inhibition of cell viability on RAW264.7 osteoclast precursor cells via combined treatment with PL and ZA. (a and b) The growth suppression of RAW264.7 cells after individual treatment with PL (a) or ZA (b) for 24, 48, 72 and 96 h. (c) Combined cytotoxicity after administration with varying ZA concentrations and PL at 0, 2.5, 5, 10 and 20 μM for 24, 48, 72 and 96 h. The fold changes were compared between individual ZA treatments with combinatory treatments. (d) Combined cytotoxicity after administrations with varying PL concentrations and ZA at 0, 12.5, 25, 50 and 100 μM for 24, 48, 72 and 96 h. The fold changes were compared between individual PL treatments with combinatory treatments. (e) Computerized analysis from the Compusyn software demonstrated the synergistic effect of PL with ZA with Fa-CI and Fa-DRI plots, using the method based on the median effect principle of Chou and Talalay. The data are presented as the means±S.D. All experiments were repeated at least three times (*significant difference at P<0.1; **significant difference at P<0.05)