Figure 4.

Elevated hepatic lipogenesis and SREBP signalling in FAIM-deficient mice. Metabolomics analysis of the ratio of hepatic unsaturated (a and b) fatty acids in WT and FAIM^−/− males (n=3) fed with NCD at 14 weeks of age. Data are expressed as mean±S.E.M. *P<0.05, **P<0.01 versus the corresponding WT value. (c and d) Protein and mRNA expression of SCD-1, SREBP-1(125 kDa, precursor; 68 kDa, mature) ACCα and FAS in liver tissues of WT and FAIM^−/− males fed with NCD at 14 weeks of age (n=3, **P<0.01). (e and f) Protein and mRNA expression of SREBP-2 and HMGR in liver tissues of WT and FAIM^−/− males (n=3, **P<0.01). WT and FAIM^−/− mice were 14 weeks of age on NCD