Figure 5.

Dyslipidaemia, hyperglycaemia and hyperinsulinemia in FAIM-deficient mice. (a) Fasting serum concentrations of triglyceride (TG), total cholesterol (TC) and free fatty acid (FFA) in WT and FAIM^−/− males (n=3) fed with NCD at 17 weeks of age. (b) Relative mRNA expression of ApoB, ApoE and LDL receptor (Ldlr) in WT and FAIM^−/− males (n=3) fed with NCD at 17 weeks of age. Data are expressed as mean±S.E.M. (n=3) *P<0.05, **P<0.01 versus corresponding WT groups. Fasting serum glucose (c) and insulin (d) levels in WT and FAIM^−/− males fed with NCD at 17 weeks of age (n=3). (e) Representative immunohistochemical staining of insulin in the pancreas of WT and FAIM^−/− males (n=3) at 14 weeks of age. Scale bar, 100 μm. (f) Insulin production in isolated islets from WT and FAIM^−/− males (n=3) at 14 weeks of age treated with glucose (11  mM) for 2 h in culture media