Figure 2.

miR-27a affects the kinetics of emission/release of DAMPs upon chemotherapeutic-mediated ICD. (a) Kinetics of induction of ATP secretion in HCT116 CRTL, miR27a_KD and miR27a_OE cells exposed to MTX (1 μM) or OXP (100 μM). (b) Immuno-detection of intracellular and extracellular HMGB1 in HCT116 CRTL, miR27a_KD and miR27a_OE cells; the quantification of the bands is illustrated in the corresponding histograms. (c) Time-course of HMGB1 secretion upon exposure of HCT116 CRTL, miR27a_KD and miR27a_OE cells to either MTX or OXP quantified as reported in the histograms in (d). All values are mean±S.D. of three independent experiments. *P⩽0.05; **P⩽0.01 (two-tailed Student's t-test)