Figure 3.

miR-27a impairs the kinetics of apoptosis execution in drug-induced ICD. (a) Western blot analysis of the apoptosis markers, PARP and caspase 3, in time-course experiments of HCT116 CRTL, miR27a_KD and miR27a_OE cells treated or not with mitoxantrone (1 μM). β-Actin was used as loading control and quantification of the cleaved protein forms is reported in (b). (c) Flow cytometry analysis of pre-apoptotic (annexin V-PE⁺ and 7-AAD^-) and apoptotic (annexin V-PE⁺ and 7-AAD⁺) HCT116 CRTL, miR27a_KD and miR27a_OE cells treated with OXP (100 μM) in kinetics experiments. One representative experiment out of three is shown