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. 2016 Feb 25;7(2):e2117. doi: 10.1038/cddis.2016.30

Figure 3.

Figure 3

NRG1 has attenuating effect against oligomeric Aβ1-42-induced reduction of neurite outgrowth. (a) Representative images of neurons stained with anti-tau antibody and visualized with FITC-coupled secondary antibody are shown. Primary hippocampal neurons were treated with 250 nM oligomeric Aβ1-42 (at DIV 13) either alone or with PBS or NRG1 (at DIV 14). The neurons were fixed and stained with anti-tau antibody, and visualized with FITC-coupled secondary antibody. Scale bar, 100 μm. (b) The values of neurite outgrowth are shown as the percentage of the control group, n=6, **P<0.01, #P<0.05. Neurite outgrowth in the neurons exposed to oligomeric Aβ1-42 (250 nM) for 4 days was reduced significantly by 41.3%. (253.7±23.7 μm, n=6, **P<0.01), compared with the PBS-treated controls (363.4±10.0 μm, n=6). Co-treatment with 10 nM NRG1 for 3 days restored neurons from oligomeric Aβ1-42-induced reduction of neurite outgrowth to 350.7±20 μm (#P<0.05, n=6)