Skip to main content
. 2016 Feb 25;7(2):e2119. doi: 10.1038/cddis.2016.32

Figure 4.

Figure 4

MAPK11 is the most significant regulator of DKK-1 mRNA expression in the p38 MAPK family. (a) PC3 cells underwent siRNA transfection targeting p38 MAPK family members, MAPK11, MAPK12 and MAPK14. For each MAPK member, three individual siRNAs were assessed. Twenty-four hours post transfection, DKK-1 mRNA expression was analyzed by qRT-PCR. Confirmation of MAPK knockdown is shown at the protein expression level as assessed by western blot. The three p38 MAPK blots (MAPK) are arranged linearly and correlate to each specific MAPK isoform antibody, the name of which appears below each respective blot. (b) The siRNA most effective at suppressing DKK-1 for each MAPK family member (MAPK11; si83, MAPK12; si66 and MAPK14; si86) was selected to assess for any further suppression when transfected in combination. Secreted DKK-1 protein levels in PC3 cell supernatant were assessed 48 h post transfection by ELISA. Western blots of MAPK family members were also performed to confirm knockdown at the level of protein expression. mRNA expression data of N=4 are shown as a percentage of the control, scrambled siRNA-transfected group and results are shown as the mean±S.D. (*P<0.05; **P<0.01, ***P<0.001)