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. 2016 Feb 25;7(2):e2121. doi: 10.1038/cddis.2016.34

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Copyright © 2016 Macmillan Publishers Limited

Cell Death and Disease is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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GUV budding upon incubation with Bcl-2 proteins. (a) Snapshots of vesicle budding from kinetic experiments. GUV mimicking the MOM composition labeled with DiI incubated with 10 nM cBid and 20 nM Bax. Scale bar: 20 μm. (b) Changes in GUV size upon 90 min incubation with the indicated proteins at 22 °C. (c) Exemplary fluorescence intensity trace of a control sample and a sample incubated with 10 nM cBid and 20 nM Bax. The background count rate is <3 kHz. Fluorescent bursts >10 times brighter as the background count rate are considered as diffusing vesicles. (d) Number of peaks detected during 600 s (with count rates >29 kHz) after 90 min incubation with the indicated proteins at 22 °C. Error bar present the S.D. (N=3). (e) Histogram of the different peak intensities describes in (d). The color code is the same as in (d)