Skip to main content
. 2016 Apr 15;193(8):898–909. doi: 10.1164/rccm.201502-0407OC

Figure 8.

Figure 8.

Hematopoietic stem cell (HSC) and progenitor cell functions were altered in the mutant (Mut) mice with pulmonary arterial hypertension. Flow cytometric analysis of cell populations was done in the bone marrow (BM) of Mut and control (Ctrl) mice. (A) CD133+ progenitor cells, (B) Lin–Sca-1+c-kit+ (LSK) cells, (C) HSCs (LSK/CD48–CD150+), and (D) multipotent progenitors (MPPs) (LSK/CD34+Flt3high). (E) BM cells from Mut mice produced more colonies in methylcellulose colony-forming assays than BM cells from Ctrl mice. (F) Mut BM cells have higher donor reconstitution units (RU) in long-term competitive transplantation assays than control BM cells. Data are shown as box-and-whisker plots, with whiskers indicating Tukey whiskers. An α level of 0.05 was chosen, and P values less than 0.05 were considered statistically significant. All P values were two-tailed. Analyses were performed with Prism 5 for Mac OS X (GraphPad Software Inc., La Jolla, CA).