Figure 2.

Susceptibility of immunocompetent mice to P. aeruginosa UNC-D respiratory challenge. BALB/c mice (n = 8) were challenged with 10^7.84 (blue), 10^7.59 (green), 10^7.34 (red) or 10^7.09 (black) CFU of P. aeruginosa UNC-D via IMIT and (A) monitored for survival. MTD indicated in parenthesis; U = undefined. (B) At the time of euthanasia, lungs were harvested and severity of lung pathology was scored (ANOVA with Tukey: * = P < 0.05; *** = P < 0.001). Solid circles represent animals that survived the entire course of infection. Open circles represent moribund animals that were euthanized. Bar represents the mean. (C) Comparison of lung pathology between animals that met euthanasia criteria [E, open circles] or survived [S, solid circles] to the end of the study (Unpaired student's t-test: ***P < 0.001). (D) Representative lungs (H&E stain) from each group. Data are representative of two independent experiments.