Figure 6.
Susceptibility of leukopenic mice to P. aeruginosa UNC-D respiratory challenge. Cy-treated BALB/c mice (n = 8) were inoculated with 105.58 (blue), 105.34 (green), 104.34 (red) or 103.34 (black) CFU of P. aeruginosa UNC-D via IMIT and (A) monitored for survival. MTD indicated in parenthesis; U = undefined. (B) At the time of euthanasia, lungs were harvested and severity of lung pathology was scored (ANOVA with Tukey: * = P < 0.05; ** = P < 0.01). Solid circles represent animals that survived the entire course of infection. Open circles represent moribund animals that were euthanized. X represents animals that succumbed to infection and tissues were not harvested. Bar represents the mean. (C) Representative lungs (H&E stain) from each group. (D) Comparison of endpoint lung bacterial burden of leukopenic (Leuko) and immunocompetent (BALB/c) receiving an ∼LD100 of UNC-D (105.58 and 107.84 CFU, respectively). Data are representative of two independent experiments.