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. 2016 Apr 27;10(Suppl 1):11–17. doi: 10.4137/GRSB.S29414

Figure 3.

Figure 3

miR-548ar interacts with NEAT1. (A) Predicted miRNAs of seed sequences targeting NEAT1 by RNAhybrid. (B) qRT-PCR for NEAT1 RNA levels in MCF-7 cells transiently transfected with synthesized microRNA mimics (20 nM). Findings are the results of three independent experiments and presented as mean ± SEM. (C) Relative expression of NEAT1 after MCF-7 cells were transfected with siAGO2 or siDicer. (D) Apoptosis of MCF-7 cells was detected by FACS staining with Annexin-V 48 hours after transfecting cells with NEAT1 siRNA.