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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: Gastroenterology. 2015 Mar 30;149(1):151–162. doi: 10.1053/j.gastro.2015.03.046

Figure 5. An acute effect of high-dose rapamycin on NHE3 expression.

Figure 5

(a–b) Western blot analysis (a) and quantification (b) of NHE3, NHERF1, total and p-Ezrin, total and p-S6, total Akt and p-Akt at Ser473. (a) Surface biotinylation assay and quantification (b) of NHE3 and NHERF1 in the membrane fraction of intestinal tissue from vehicle- and rapamycin-treated mice (*p<0.05, t-test, N=3). (c) Representative traces showing the effect of rapamycin on Na+/H+ activity in PS120 cells stably expressing NHE3 and NHERF2. Bar graph showing Vmax (d) and K(H+)i (μM) (e) in vehicle- and rapamycin-treated groups (*P<0.05, One way ANOVA with Dunnett’s post hoc test, N=3).