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. 2016 Apr 28;11(4):e0154570. doi: 10.1371/journal.pone.0154570

Fig 2. Validation of the NLR assay: analysis of the non-touch-responsive maco mutant zebrafish strain.

Fig 2

The NLR assay was successful at discriminating mutant embryos from their non-mutant siblings. The locomotor activity of 36 hpf embryos triggered by 120μM of nicotine was significantly different between mutants and non-mutant embryos (A) as demonstrated by the comparison of the average maximum speed of mutant and non-mutant embryos (B). The difference between mutants and non-mutants became progressively more apparent in 48 hpf (C and D) and 72 hpf (E and F) embryos. The three dots above the graphs in panels B, D and F represent P <0.001.