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. 2016 Apr 28;11(4):e0154570. doi: 10.1371/journal.pone.0154570

Fig 3. Spontaneous swimming of 6 dpf MeHg-exposed zebrafish eleutheroembryos and the NLR of MeHg-exposed 48 hpf embryos.

Fig 3

The spontaneous-swimming assay elucidated subtle yet significant (P<0.001) increases in the total distance travelled (mm in 5 minutes) of free swimming 6 dpf zebrafish exposed to 0.01 and 0.03μM of MeHg (A). Eleutheroembryos exposed to 0.01μM MeHg as embryos also had an increased activity (% of time active) relative to all other doses (B). The NLR assay was conducted on 48 hpf embryos exposed to 0, 0.01, 0.03 and 0.1μM; the activity curves of all MeHg-exposed embryos were compared to the control (C through E). As observed in 6 dpf eleutheroembryos, 48 hpf zebrafish embryos exposed to 0.01 and 0.03μM of MeHg exhibited an increase in distance traveled during the analysis period (F).