Fig. 3.

Western blot analysis of cPLA₂ (A), COX-1 (B), and COX-2 (C) expression in the rat gastric mucosa damaged by ethanol; the mucosal concentrations of PGE₂ (D) and LTB₄ (E) are also shown. The ethanol-administered rats showed marked increases in cPLA₂ (A), COX-1 (B), and COX-2 (C) expression in the gastric mucosa (a representative blot is shown). These increases were significantly suppressed by pretreatment with PMK-S005 (5 mg/kg) or rebamipide (50 mg/kg), which were given 1 hour before ethanol administration. The mucosal concentrations of PGE₂ (D) and LTB₄ (E) were determined 1 hour after ethanol administration using an enzyme-linked immunosorbent assay. The results are expressed as the mean±SEM from five to 10 animals per group. *p<0.05 compared with the ethanol group; ^†p<0.05 compared with the control group.